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Hawken tobacco reviews1/2/2024 moist snuff brands sold in the United States during 1997 (3). The pH, nicotine, and total moisture content in samples of the six products were analyzed at CDC using a federal standard protocol** (4). Samples were stored in their original containers at -95.8 F (-71 C) until tested. The pH was obtained by suspending 2 g of moist snuff in 10 mL distilled water. Total moisture content (water and tobacco constituents that are volatile at 211.1 F ) was obtained by calculating the weight difference in 5 g of tobacco before and after 3 hours of oven drying at 211.1 F (99.5 C). Nicotine was extracted from moist snuff by using methyl tertbutyl ether, and tobacco extracts were analyzed by gas chromatography to determine the nicotine content. The nicotine extraction and pH measurements were conducted at room temperature. The percentage of free (unprotonated) nicotine, which is dependent on the pH, was calculated according to the Henderson-Hasselbalch equation and by using a pKa value of 8.02 for nicotine (5). #Hawken chewing tobacco pouches free#įree nicotine content then was calculated by multiplying the percentage of free nicotine by the total nicotine content (percentage of free nicotine x nicotine content). The tests were not blinded to the brands being tested, and all analyses were done in triplicate. Statistical analyses were performed using Statistical Analysis System (SAS) software. The mean total moisture content ranged from 48.9% to 54.1%, except Hawken Wintergreen, which had a mean total moisture content of 24.7% the mean nicotine content varied from 7.11 mg/g to 11.04 mg/g, except Hawken Wintergreen, which had a mean nicotine content of 3.37 mg/g the mean pH varied from 5.24 (Hawken Wintergreen) to 8.35 (Kodiak Wintergreen).
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